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UID:pretalx-flowpath-2025-CEZG9R@talks.staging.osgeo.org
DTSTART;TZID=CET:20250611T153000
DTEND;TZID=CET:20250611T154000
DESCRIPTION:Biomarkers associated with the biodegradation of chlorinated et
 henes (CEs)\, such as RDase functional gene DNA\, mRNA\, and enzymes\, are
  increasingly monitored in contaminant plumes to assess reductive dechlori
 nation (RD). These biomarkers provide direct proof of RD and are used to e
 valuate biodegradation potential and possibly infer degradation rates. How
 ever\, aquifers are inherently heterogeneous\, and local hydrodynamic and 
 biogeochemical conditions likely influence the behavior and efficiency of 
 organohalide-respiring bacteria. Moreover\, identifying the key factors sh
 aping the observed spatiotemporal patterns of chemicals and biomarkers rem
 ains a challenge.\nAt the Grindsted site (Denmark)\, a 1500-m long CE plum
 e has developed from a former pharmaceutical factory. Extensive monitoring
  of both chemicals and biomarkers has been conducted at this site. To aid 
 in the data interpretation\, we developed new enzyme-based kinetics linkin
 g the reductive dechlorination of cis-1\,2-dichloroethene to vinyl chlorid
 e and ethene with the expression of the vcrA and bvcA genes in Dehalococco
 ides. These kinetics were integrated into a 1D reactive transport model si
 mulating the Grindsted plume\, also including the estimated variable flow 
 rates. Fitting the observed chemicals and biomarkers (i.e.\, functional ge
 ne DNA and mRNA) required to calibrate the kinetic parameters with a zonal
  approach.\nAs a result\, the model pointed out different ecological strat
 egies of vcrA- and bvcA-carrying Dehalococcoides\, which affected the RD e
 fficiency within the plume core. Moreover\, CE biodegradation efficiency a
 ppeared further decreased under high flow rate conditions\, because of the
  shorter residence time.\nThis modeling endeavor is the first attempt of f
 ield application and allowed enhancing the mechanistic interpretation of o
 bserved chemical and biomarker patterns in the CE plume. Consequently\, it
  proved to be a reliable support for designing of future monitoring and re
 mediation strategies.
DTSTAMP:20260427T221741Z
LOCATION:Room R3
SUMMARY:Interpreting chemical and biomarker patterns with reactive transpor
 t modeling to assess chlorinated ethene biodegradation in groundwater - Di
 ego Di Curzio
URL:https://talks.staging.osgeo.org/flowpath-2025/talk/CEZG9R/
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